Journal: Scientific Reports
Article Title: Genome mining of Streptomyces bambergiensis AC-800 unravels the biosynthetic gene cluster for inhibitors of prolyl hydroxylase fibrostatins
doi: 10.1038/s41598-025-17585-y
Figure Lengend Snippet: PKS type I gene cluster located on the giant linear plasmid pSB1: evolutionary relations with the stambomycin gene cluster of S. ambofaciens . PKS genes are numbered according to the biosynthetic order predicted for pSB1 PKS BGC by antiSMASH and experimentally verified for stambomycins. Homologous PKS genes are shaded and marked with dotted lines and those designated in blue font are unique for each cluster. Stb _PKS – PKSI of the stambomycin BGC; Sbam_PKS – PKSI of the pSB1-licated BGC in S. bambergiensis .
Article Snippet: The latter SMs were produced by Streptomyces ambofaciens ATCC 23,877 only upon overexpression of a pathway-specific regulator of the LuxR type .
Techniques: Plasmid Preparation